GSM5176089: MCF-7 E2-EHT RAC1 ChIPSeq rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RAC1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF-7 cells
cell type
Breast cancer cell line
chip antibody
Rac1 (Millipore, 05-389)
treatment
10 nM E2 + 50 uM EHT 1864
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 15 min at RT. Sonicated nuclear lysates were used in immunoprecipitation with a target antibody. Libraries were prepared using NEBNext UltraII DNA library Kit for Illumina (E7645).