Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAC1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 cells
cell type
Breast cancer cell line
chip antibody
Rac1 (Millipore, 05-389)
treatment
10 nM E2 + 50 uM EHT 1864

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 15 min at RT. Sonicated nuclear lysates were used in immunoprecipitation with a target antibody. Libraries were prepared using NEBNext UltraII DNA library Kit for Illumina (E7645).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
27274176
Reads aligned (%)
96.4
Duplicates removed (%)
8.9
Number of peaks
28423 (qval < 1E-05)

hg19

Number of total reads
27274176
Reads aligned (%)
95.6
Duplicates removed (%)
10.4
Number of peaks
28204 (qval < 1E-05)

Base call quality data from DBCLS SRA